FIG. 4.

Imprint erasure at the Snrpn and H19 imprinting control centers (ICCs) happens within 3 days in the organ culture model. (A) Bisulfite PCR of the Snrpn and H19 ICCs, as well as IAP, comparing somatic cells (left) and OG-sorted PGCs (right) from the embryo at E10.5, (B) E13.5, (C), and after 3 days of AGM organ culture. Black circles, methylated cytosines; white circles, unmethylated cytosines. (D) Quantification of average cytosine methylation in OG-sorted PGCs at the Snrpn ICC from the in vitro cultured AGM at days 1–3. (E) Quantification of average cytosine methylation in OG-sorted PGCs at the H19 ICC from the in vitro cultured AGM at days 1–3. * Refers to statistical significance P<0.05.