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. 2015 Mar 6;24(13):1536–1545. doi: 10.1089/scd.2014.0410

FIG. 5.

FIG. 5.

Imprint erasure dynamics at the Snrpn and H19 ICCs in PGCs after treatment with PI3-kinase inhibitor. (A) Cell cycle analysis of Mvh-positive PGCs and Mvh-negative somatic cells using Edu incorporation of in vitro cultured AGMs from days 1–3 of culture with DMSO or (B) 10 μM LY294004. Somatic cells (red), Mvh-positive PGCs (blue), Edu incorportation (APC), DNA content (PE-Cy5). (C) Percentage of viable cells between DMSO (vehicle) and LY294004-treated samples. (D) Percent cytosine methylation at the Snrpn ICC plotted as a function of the cell number during days 1–3 of AGM cell culture in the presence of DMSO or (E) LY294004. (F) Percent cytosine methylation at the H19 ICC plotted as a function of the cell number during days 1–3 of AGM cell culture in the presence of DMSO or (G) LY294004.