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FGF2 release by MSC and SB623 in high cell density/hypoxia/insufficient nutrients cultures. MSC or SB623 were plated in round 96-well plates at 1×106 cells/mL, 350 μL/well, and tightly closed to prevent gas exchange. At the indicated time points, the well contents were collected. FGF2 (A) and LDH activity (B) were measured in both culture medium (CM) and cells (intracellular) after releasing intracellular contents by freeze/thaw treatment. The results are expressed per million cells.
