Table 1.
Detection of Various Mesenchymal Stromal Cells Factors in Conditioned Media and Freeze/Thaw Extracts (E0)
| CM | E0 | Difference (E0/CM) | |||
|---|---|---|---|---|---|
| Average (ng/106 cells) | CV (%) | Average (ng/106 cells) | CV (%) | Fold | |
| FGF2 | 0.017 | 94 | 3.9 | 64 | 230 |
| FGF1 | LLD | — | 1.1 | 49 | — |
| VEGF | 0.4 | 121 | 0.002 | 23 | 0.005 |
| MCP1 | 0.003 | 85 | 0.001 | 89 | 0.3 |
Amounts of factors produced by 1 million MSC. Average measurements of seven cell lots are shown for FGF2 and of two cell lots are shown for other factors.
CM, conditioned media; FGF, fibroblast growth factor; VEGF, vascular endothelial growth factor; LLD, lower limit of detection; CV, coefficient of variation; MSC, mesenchymal stromal cells.