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Challenges and strategy for quantifying cardiomyocyte renewal. (A) Cardiomyocytes have the potential to undergo “nonproductive” DNA replication. (B) Continuous pulsing of BrdU has the potential to label the cardiomyocyte generation regardless of the cellular source. (C) Isolation of nuclei allows accurate quantification of BrdU labeling of cardiomyocyte nuclei and discrimination of BrdU incorporation due to polyploidy. Color images available online at www.liebertpub.com/scd
