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. 2015 Mar 6;24(14):1703–1711. doi: 10.1089/scd.2014.0553

FIG. 5.

FIG. 5.

Cells from naïve mouse lungs were analyzed by flow cytometry. CD45+ Lin−/lo c-kithi T1/ST2+ CD16/32int integrin β7hi cells expressed FcɛRI and had a low SSC light profile, suggesting that these cells were MCp. CD45+ Lin−/lo c-kithi T1/ST2+ CD16/32int integrin β7−/lo/int cells also expressed FcɛRI, but had a high SSC light profile, indicating that that they were mature mast cells. Pooled cells from three to four mice were analyzed. The graphs are derived from one out of two similar experiments. The frequency of the gated cells in each graph was calculated by dividing the gated cell numbers with the number of CD45+ lung cells.