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. 2015 Jul 13;5:12006. doi: 10.1038/srep12006

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Schematic drawing of the model system for SNARE mediated membrane fusion based on pore-spanning membranes. SNARE proteins are shown in red (syntaxin 1A), green (SNAP25) and blue (synaptobrevin 2). Membranes are depicted as thick orange lines. (B) Scanning electron micrograph of a porous silicon nitride substrate. Scale bar: 3 μm. (C) Pore-spanning membrane patch obtained from spreading a giant unilamellar vesicle composed of DOPC/POPE/POPS/cholesterol (5:2:1:2) and doped with 1 mol % Oregon Green DHPE on a gold/6-mercapto-1-hexanol-functionalized substrate. Scale bar: 20 μm.