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. 2015 Jul 13;5:12132. doi: 10.1038/srep12132

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Human brain pericytes were treated with vehicle or 0.01–10 ng/mL IL-1β for 24 hours. Representative immunocytochemistry images of C/EBPδ are shown (a). The percentage of cells positive for nuclear C/EBPδ was determined by immunocytochemistry (b) and C/EBPδ intensity was analysed by western blotting (c,d). Blots are cropped to improve clarity. Full-length blots are presented in Supplementary Figure S2. Human brain pericytes were treated with 10 ng/mL IL-1β for 0–48 hours. The percentage of C/EBPδ positive cells was determined by immunocytochemistry (e) and C/EBPδ intensity was analysed by western blotting (f,g). Data is displayed as mean ± SEM from three independent experiments. ^* = p < 0.05 compared to vehicle control, ^** = p < 0.01 compared to vehicle control, ^*** = p < 0.001 compared to vehicle control. Scale bar = 100 μm.