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. 2015 Jul 13;5:12132. doi: 10.1038/srep12132

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Human brain pericytes were transfected with 50 nM of control or C/EBPδ siRNA for 48 hours. Following transfection, cells were treated with vehicle or 10 ng/mL IL-1β for 4–48 hours. Representative immunocytochemistry images of C/EBPδ with treatments are shown (a) and the percentage of cells positive for nuclear C/EBPδ was quantified (b). RNA was extracted following a six hour treatment with IL-1β and C/EBPδ transcript expression was determined via RT-qPCR (c). Protein was extracted following a 24 hour treatment with IL-1β and C/EBPδ expression determined by western blotting (d,e). Blots are cropped to improve clarity. Full-length blots are presented in Supplementary Figure S2. ^*** = p < 0.001. Scale bar = 100 μm.