Static and cidal curve with different concentrations
of compound 27. (A) Compound 27 (1×
and 10× MIC concentration) were incubated with S. aureus for 24 h, and at regular time points, aliquot was taken and CFU
was calculated. In vivo antimicrobial activity of
the compound 27. (B) BALB/C mice were initially made
neutropenic by injecting cyclophosphamide (150 mg/kg of body weight),
followed by S. aureus ATCC 29213 injection. After
3 h of post infection, compound 27 and vancomycin at
15 mg/kg of body weight was injected into mice twice at an interval
of 3 h between injection. After 24 h, the mice were sacrificed and
the organs (spleen, kidney, liver) were crushed before plating them
on the TH plates for CFU determination. The data from three independent
experiments were pooled, and the P-value was determined
using the ANOVA on ranks by using Sigma stat software (***P < 0.001 and **P < 0.05).