. Author manuscript; available in PMC: 2016 Jan 9.

Published in final edited form as: Nat Commun. 2015 Jul 9;6:7449. doi: 10.1038/ncomms8449

Table 1. Drug loading properties of nanocarrier library.

The drug loading capacity, efficiency, CMC and particle sizes of the telodendrimer nanocarrier library made from eight selected DBMs.

DBM (rank-ing)	DOX	PEG^5KDBM₈ (G1)			PEG^5KCA₄DBM₄ (G2)			PEG^5KCA₄-L-DBM₄ (G3)
DBM (rank-ing)	DOX	CMC (μM)	Size (nm)	DOX LE (%)^a LE₀ - LE₁ - LE₇	CMC (μM)	Size (nm)	DOX LE (%)^a LE₀ - LE₁ - LE₇	CMC (μM)	Size (nm)	DOX LE (%)^a LE₀ - LE₁ - LE₇
AQ(1)	0	2.84	PPT	N/A	2.1	MP	N/A	2.36	MP	N/A
	10%	-	PPT	49- -	-	24±7	100-100-91	-	27±7	100-94-100
	20%	-	-	-	-	25±6	100-87-93	-	29±9	100-96-100
	30%	-	-	-	-	20 ±5	100-84-61	-	30±8	100-71-58
	40%	-	-	-	-	MP	94-54-66	-	-	-
Rh(2)	0	ND	PPT	N/A	0.93	17±5	N/A	2.7	32±8	N/A
	10%	-	PPT	-	0.89	20±5	100-100-100	1.1	30±8	100- 100-100
	20%	-	-	-	-	24±7	100-98-100	-	28±7	100-78-86
	30%	-	-	-	-	19±4	100-85-95	-	21±5	100-56-64
	40%	-	-	-	-	15±4	100-75-97	-	-	-
LiCA(4)	0	1.18	36±11	N/A	1.45	17±4	N/A	1.2	17±4	N/A
	10%	-	350/49 ^b	100-100-87	-	14±3	100-100-57		15±3	100- 93-59
	20%	-	59/33 ^b	99-70-52	-	16±4	100-64-29		Mp	98-46-19
Cho(5)	0	0.89	PPT	N/A	0.75	17±4	N/A	1.2	18±4		N/A
	10%	-	MP	100-100-87	-	39±12	100-100-42	-	23±7	100-84-33
	20%	-	-	-	-	41±16	100-72-30	-	24±8	93-63-18
Co(8)	0	2.59	35 ± 12	N/A	1.99	14 ± 4	N/A	1.7	36 ± 13	N/A
	10%	-	28 ± 9	100-100-92	-	13 ± 2	100-97-97	-	36 ± 13	100-100-85
	20%	-	27 ± 8	100-94-73	-	385/133 ^b	99-82-84	-	38 ± 13	100-68-80
	30%	-	32 ± 10	100-75-65	-	-	-	-	342/32 ^b	99-60-74
Phe (11)	0	1.77	42±19	N/A	2.86	13±3	N/A	2.9	103±39	N/A
	10%	-	34±9	100-100-92	-	185/14	100-75-36	-	240/22 ^b	100-91-36
	20%	-	34±14	100- 95-100	-	-	-	-	17±4	92-45-13
	30%	-	29 ±8	100-53-40	-	-	-	-	-		-
VE(12)	0	2.5	MP	N/A	1.6	MP	N/A	1.4	25±6	N/A
	10%		MP	78-64-55	-	38±15	100-90-65	-	23±6	100-85-61
	20%		N/A	N/A	-	36±12	96-50-34	-	24±6	84-46-25
C17 (13)	0	1.66	PPT	N/A	1.33	13±4	N/A	2.7	MP	N/A
	10%	-	408/61	86- -	-	14±2	100-92-32	-	196±99	100-92-43
	20%	-	-	-	-	14±3	85-61-24	-	MP	90-44-17

Note:

DOX loading efficiency (LE) were measured by fluorescence (λ_ex=470 nm, λ_em=590 nm) measurements of samples before and after spin at the speed of 13,000g for 5 min; The drug loading efficiencies were measured right after drug loading process (LE₀) and after a storage at 4 °C overnight (LE₁) or after a storage at room temperature for one week (LE₇). Therefore drug loading efficiencies were presented as LE₀ - LE₁ - LE₇.

Two peaks were detected in DLS particle size analysis; N/A: not applicable; ND: not detected; MP: multiple peaks; PPT: precipitation; -: not tested