Figure 6. GalT–mCherry puncta localize to lysosomes.

(a) Hela cells expressing GalT–oxBFP (upper panels) or GalT–mCherry (lower panels) were fixed and stained with the GC marker anti-GM130. Co-localization was observed for GalT–oxBFP, but cells expressing moderate levels of GalT–mCherry exhibit both GC co-localization and bright puncta throughout the cell. (b) GalT–mCherry expressing HeLa cells fixed and stained for the GC and intermediate compartment marker β COP. The red puncta do not co-localize with the green β COP puncta. (c,d) HeLa cells co-expressing GalT–moxGFP and GalT–mCherry show co-localization in the GC structure, but GalT–moxGFP does not localize to red puncta. (d) Pretreatment with NH₄Cl for 3 h leads to substantial co-localization of GalT–moxGFP with red puncta. (e) GalT–mcherry expressing HeLa cells were fixed and stained for the lysosomal marker anti-LAMP1. Several red puncta co-localize with the LAMP1 positive structures. Scale bar, 10 μm.