Figure 1. Cep63 deficiency leads to growth defects and microcephaly.

(a) Weights of newborn mice (p2) of the indicated genotype (Cep63^{+/+, +/T} n = 11, Cep63^T/T n = 5 pups). (b) Weight of p30 and p60 mice of the indicated sex and genotype (n = 13, 11, 16, 14, 13, 13, 18, 14 animals, respectively). (c) Examples of female littermate Cep63^+/+and Cep63^T/Tmice at p60. (d) Examples of p2 dissected brains of the indicated genotype with the relative cerebral cortex area compared. (e) Quantitative real-time PCR of the indicated genes using cDNA prepared from the cortex of p2 animals. Levels of mRNA expression relative to WT littermates is graphed using B2M and ACTB as a reference. (n=3 animals per genotype). (f) H&E stained coronal sections of Cep63^+/+and Cep63^T/T cortices from p2 animals with approximate area of higher magnification indicated (top panels). Higher magnification panels of the motor cortex stained with H&E (bottom panels, scale bar = 0.2 mm). (g) Quantification of p2 cortical thickness from the indicated genotypes and area (2 hemispheres per brain section were averaged, n = 3 animals is graphed). (h) Kaplan-Meier curve of mouse survival. No significant differences (Log-rank test) were observed over 600 days. The number of animals per genotype is indicated. In panels 1a and 1b, the median (thick line) with the 1^st and 3^rd quartiles of the boxplot (thin lines) are indicated. In 1e and 1g graphics with error bars represent the average plus standard deviation. Asterisks denote statistical significance (n.s.= not significant, * = p-value <0.05, ** = p-value < 0.01, *** = p-value < 0.001) determined by the unpaired two-way Student’s T-test (1a, 1g) or the unpaired two-way Wilcoxon rank sum test (1b).