Table 1.
Metabolites that distinguish colon cancer cell lines from ovarian cancer cell lines
| Metabolic group/metabolites | Ovarian-colon | Levels in ovary | HCT15_HCT116 | SKOV3_OVCAR3 |
|---|---|---|---|---|
| p value | p value | p value | ||
| Amino acid metabolism | ||||
| Citrulline | 5.48 × 10−7 | Higher | 0.0433 | 0.0003 |
| Cystathionine | 5.96 × 10−11 | Higher | 0.1051 | 0.0676 |
| Dimethylarginine (SDMA + ADMA) | 2.08 × 10−7 | Higher | 0.2176 | 0.0545 |
| Histidine | 1.61 × 10−7 | Higher | 0.1230 | 0.6965 |
| Isoleucine | 1.13 × 10−9 | Higher | 0.2475 | 0.0003 |
| Methionine | 5.96 × 10−11 | Higher | 0.7959 | 0.0343 |
| Methionine sulfoxide | 1.79 × 10−9 | Higher | 0.0011 | 0.0021 |
| N-acetylmethionine | 1.79 × 10−9 | Higher | 0.4813 | 1.0000 |
| N-acetylputrescine | 1.54 × 10−7 | Lower | 0.2176 | 0.2133 |
| N6-acetyllysine | 4.17 × 10−10 | Higher | 0.0015 | 0.0676 |
| Phenylacetylglycine | 1.52 × 10−7 | Lower | 0.1431 | 0.0043 |
| Phenylalanine | 5.96 × 10−11 | Higher | 0.5288 | 0.0031 |
| Tyrosine | 5.96 × 10−11 | Higher | 0.2799 | 0.0434 |
| Valine | 5.96 × 10−11 | Higher | 0.7959 | 0.0009 |
| Carbohydrate | ||||
| Isobar: ribulose 5-phosphate, xylulose 5-phosphate | 0.000172 | Higher | 0.0412 | 0.0003 |
| Mannose-6-phosphate | 1.54 × 10−7 | Higher | 0.6230 | 0.0005 |
| UDP-glucuronate | 5.96 × 10−11 | Higher | 0.0052 | 0.3599 |
| Cofactors and vitamins | ||||
| Thiamin (Vitamin B1) | 1.54 × 10−7 | Higher | 0.0101 | 0.0044 |
| Energy | ||||
| Fumarate | 2.08 × 10−7 | Higher | 0.0068 | 0.0343 |
| Malate | 1.63 × 10−5 | Higher | 0.6305 | 0.1728 |
| Succinate | 5.96 × 10−11 | Higher | 0.6842 | 0.0003 |
| Lipids | ||||
| 1-Arachidonoylglycerophosphoethanolamine | 1.13 × 10−9 | Higher | 0.2475 | 0.1011 |
| 1-Docosahexaenoylglycerophosphoethanolamine | 3.77 × 10−5 | Higher | 0.9118 | 0.0434 |
| 1-Palmitoylglycerophosphoethanolamine | 0.000128 | Higher | 0.1230 | 0.0085 |
| 1-Palmitoylglycerophosphoinositol | 1.16 × 10−8 | Higher | 0.7959 | 0.4598 |
| 1-Stearoylglycerophosphoethanolamine | 5.49 × 10−6 | Higher | 0.0892 | 0.0044 |
| 1-Stearoylglycerophosphoinositol | 1.24 × 10−5 | Higher | 0.0756 | 0.2743 |
| 2-Arachidonoylglycerophosphoethanolamine | 8.28 × 10−9 | Higher | 0.9705 | 0.0085 |
| 2-Docosahexaenoylglycerophosphoethanolamine | 1.15 × 10−5 | Higher | 0.0433 | 0.0031 |
| 2-Linoleoylglycerophosphoethanolamine | 1.75 × 10−6 | Higher | 0.5966 | 0.1728 |
| Butyrylcarnitine | 4.53 × 10−6 | Lower | 0.2176 | 0.0003 |
| Deoxycarnitine | 0.000023 | Lower | 0.0003 | 0.5726 |
| Dihomo-linoleate (20:2n6) | 5.96 × 10−11 | Higher | 0.6842 | 0.2370 |
| Dihomo-linolenate (20:3n3 or n6) | 5.96 × 10−11 | Higher | 0.1431 | 0.0062 |
| Glycerol 3-phosphate (G3P) | 5.96 × 10−11 | Higher | 0.5787 | 0.0266 |
| Linoleate (18:2n6) | 5.96 × 10−11 | Higher | 0.6305 | 0.0831 |
| Mead acid (20:3n9) | 5.96 × 10−11 | Higher | 0.2799 | 0.0021 |
| Myo-inositol | 5.96 × 10−11 | Higher | 0.6842 | 0.0062 |
| Scyllo-inositol | 5.96 × 10−11 | Higher | 0.7959 | 0.6965 |
| Nucleotide | ||||
| Guanosine 5′- monophosphate (5′-GMP) | 6.89 × 10−7 | Higher | 0.0185 | 0.6334 |
| Peptide | ||||
| Alanylleucine | 1.79 × 10−9 | Higher | 0.3527 | 0.0003 |
| Glycylglycine | 5.96 × 10−11 | Higher | 0.0524 | 0.2743 |
| Glycylleucine | 5.96 × 10−11 | Higher | 0.0007 | 0.1220 |
| Leucylaspartate | 5.96 × 10−11 | Higher | 0.7394 | 0.0545 |
| Leucylglutamate | 2.28 × 10−7 | Higher | 0.2475 | 0.0005 |
| Leucylglycine | 5.78 × 10−9 | Higher | 0.2176 | 0.0205 |
| Phenylalanylglycine | 5.96 × 10−11 | Higher | 0.0005 | 0.0044 |
| Phenylalanylserine | 7.22 × 10−8 | Higher | 0.1051 | 0.0031 |
| Prolylalanine | 2.68 × 10−9 | Higher | 0.5288 | 0.6334 |
| Prolylglutamate | 5.96 × 10−11 | Higher | 0.0068 | 0.0545 |
| Prolylglycine | 5.96 × 10−11 | Higher | 0.2799 | 0.0676 |
| Serylleucine | 3.99 × 10−9 | Higher | 0.1903 | 0.0031 |
| Seryltyrosine | 1.61 × 10−7 | Higher | 0.4359 | 0.9654 |
| Tyrosylglycine | 2.38 × 10−10 | Higher | 0.0029 | 0.0044 |
| Valylaspartate | 9.51 × 10−8 | Higher | 0.0433 | 0.0031 |
| Valylglycine | 7.15 × 10−10 | Higher | 0.0355 | 0.8968 |
| Valylleucine | 1.16 × 10−8 | Higher | 0.3527 | 0.0014 |
| Xenobiotics | ||||
| Erythritol | 4.07 × 10−8 | Higher | 0.1230 | 0.0009 |
| Unknown | ||||
| X – 11,677 | 8.28 × 10−9 | Higher | 0.0021 | 0.1728 |
| X − 11,687 | 5.96 × 10−11 | Higher | 0.0068 | 0.8968 |
| X − 11,787 | 2.71 × 10−5 | Lower | 0.3527 | 0.0831 |
| X − 14,056 | 8.28 × 10−9 | Higher | 0.4813 | 0.7618 |
| X − 14,603 | 4.11 × 10−6 | Higher | 0.0068 | 0.0067 |
| X − 14,949 | 7.06 × 10−5 | Lower | 0.0007 | 0.0545 |
| X − 15,546 | 5.96 × 10−11 | Higher | 0.0089 | 0.6334 |
| X − 17,115 | 1.49 × 10−7 | Higher | 0.0866 | 0.6965 |
| X − 19,411 | 1.53 × 10−7 | Higher | 0.2116 | 0.2370 |
Sixty-seven metabolites were identified that significantly differentiated colon cancer cell lines from ovarian cancer cell lines according to following criteria: significant difference between ovarian and colon cell lines (p < 2.22 × 10−4 for comparison of HCT116 + HCT15 vs. OVCAR3 + SCOV3), but no differences between cell lines of the same origin, (p > 2.22 × 10−4 for HCT116 vs. HCT15 and OVCAR3 vs. SCOV3). P values were calculated with metaP server using the Mann–Whitney test.