Table 3.
Biological specimen collection schedule and planned laboratory analyses in the Maternal Vitamin D for Infant Growth (MDIG) trial in Dhaka, Bangladesh
| Maternal-fetal | Infant | Father | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Baseline | 30 wk gestation | Delivery | Cord blood | 3 mo postpartum | 6 mo postpartum | 3 mo | 6 mo | 12 and 24 mo | |||
| Blood/serum/plasma | Calcium | Ra | R | R | R | R | R | R | R | ||
| Phosphate, alkaline phosphatase, creatinine | Sa | ||||||||||
| 25(OH)D | R | S | S | S | S | S | |||||
| 1,25(OH)2D, whole/bioactive PTH(1–84), C-terminal PTH(73–84), intact PTH, FGF-23 | S | S | S | S | S | ||||||
| PTHrP | S | S | S | S | S | S | S | ||||
| IGF-1, IGFBP-1, IGFBP-3 | S | S | |||||||||
| Ferritin | S | ||||||||||
| Retinol, folate, cadmium | S | ||||||||||
| C-reactive protein, IL-6, IL-8, TNF-α | S | S | |||||||||
| Epigenetic studies | S | S | S | ||||||||
| Collection for future analyses | R | R | R | R | R | R | R | R | R | R | |
| Placenta | Epigenetic studies | R | |||||||||
| Urine | Calcium:creatinine ratio | R | S | ||||||||
| Calcium, phosphate, fluoride, cyclic AMP | S | ||||||||||
| Collection for future analyses | R | R | R | ||||||||
| Breast milk | PTHrP | S | S | ||||||||
| Collection for future analyses | R | R | |||||||||
| Nasal swabb | PCR for respiratory viruses | Rb | Rb | ||||||||
| PCR for Streptococcus carriage density | Rb | Rb | |||||||||
FGF fibroblast growth factor, IGF insulin-like growth factor, IGFBP insulin-like growth factor-binding protein, IL interleukin; PTHrP parathyroid hormone–related protein, R routine, S subset, TNF tumor necrosis factor
aRoutine analyses to be performed in all specimens collected; subset analyses to be performed in a sub-set of stored specimen aliquots
bNasal swabbing is performed at any time from 1 wk to 6 mo of age if criteria for acute respiratory infection are met