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. Author manuscript; available in PMC: 2015 Jul 13.
Published in final edited form as: Hepatology. 2008 May;47(5):1437–1445. doi: 10.1002/hep.22197

Fig. 1.

Fig. 1

(A) Immunoprecipitation of Huh7.5.1-secreted ApoB followed by anti-HCV core staining (coimmunoprecipitation). (B) Cell culture secretion of ApoB, HCV-positive strand RNA, and HCV core protein in JFH-1–infected Huh7.5.1 cells in response to oleate, insulin, and brefeldin A. The secretions of ApoB, HCV RNA, and HCV core protein are significantly up-regulated by oleate and down-regulated by insulin in a dose-dependent manner. Brefeldin A, which blocks Golgi-dependent secretion of proteins, significantly inhibits the secretion of ApoB, HCV RNA, and HCV core. Cell viability for all conditions was greater than 90%. (C) Infectivity of cell culture supernatant assessed by colony formation on naïve Huh7.5.1 cells: oleate (0.8 mM), insulin (500 U/L), brefeldin A (2.5 µg/mL), and naringenin (200 µM). **P < 0.01.