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. Author manuscript; available in PMC: 2016 Aug 7.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jun 6;463(4):888–893. doi: 10.1016/j.bbrc.2015.06.029

Figure 2.

Figure 2

Inhibitory activity of 6 known kinase inhibitors in the MEKK2 transphosphorylation slot blot assay. (A) MEKK2 enzyme reactions using MKK6 as a substrate were stopped, transferred to a membrane via a slot blot apparatus and probed with phosphoMKK6-specific antibody detected by chemiluminescence. A representative slot blot image is shown with bands labeled as max (DMSO) and min (no MEKK2) controls and compound serial dilution. (B) The resultant digital image band intensities were converted to numerical data for IC50 determination. The compounds were tested at the indicated concentrations with the resultant raw signal normalized to maximum (DMSO only) and minimum (no MEKK2) controls, representing 100% and 0% enzyme activity, respectively. Data points represent the average of two determinations per concentration and error bars represent standard deviation (SD). This plot is representative of two independent experiments.