. Author manuscript; available in PMC: 2015 Jul 13.

Published in final edited form as: Immunol Lett. 2008 Oct 7;121(2):87–92. doi: 10.1016/j.imlet.2008.09.001

Table 1. T cells expressing Foxp3 and folate receptor 4 in lung and spleen (Mean±SEM).

Expression of Foxp3 and folate receptor 4 proteins in pulmonary and splenic CD4⁺CD25⁺ T cells of OVA-sensitized and challenged C57BL/6 mice treated with antibodies against TCR-γδ

Treatment	Lung				Spleen
	Foxp3⁺ cells among CD3⁺ cells		FR4⁺ cells among CD3⁺ cells		Foxp3⁺ cells among CD3⁺ cells		FR4⁺ cells among CD3⁺ cells
	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)
2ip3n + hamster IgG	4.6±0.4	5.9±0.6	22.6±3.5	27.9±1.8	0.9±0.1	43.3±10.3	26.6±0.5	1221.0±250.1
2ip3n + mAb 2.11	6.2±1.2	7.9±0.9	29.1±2.0	37.9±6.3	0.9±0.2	32.9±7.5	25.5±0.3	917.4±110.5
2ip3n + mAb UC3	5.1±0.3	17.8±8.9	31.8±2.7	100.5±40.6	1.1±0.2	27.8±4.4	24.3±0.4	706.8±203.1
	Foxp3⁺ cells among CD3⁺CD4⁺ cells		FR4 high cells among CD3⁺CD4⁺ cells		Foxp3⁺ cells among CD3⁺CD4⁺ cells		FR4 high cells among CD3⁺CD4⁺ cells
	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)
2ip3n + hamster IgG	6.7±0.5	5.6±0.6	31.1±3.2	25.6±0.6	1.6±0.1	40.8±9.8	38.4±0.7	961.0±197.0
2ip3n + mAb 2.11	8.7±0.8	7.2±0.8	36.1±2.4	30.5±4.4	1.6±0.3	29.2±5.8	37.5±0.5	693.7±78.3
2ip3n + mAb UC3	7.5±0.5	16.4±8.1	37.1±0.4	77.2±32.6	2.0±0.3	25.7±4.1	35.7±0.6	533.1±170.6
	Foxp3⁺ cells among CD4⁺CD25⁺ cells		FR4 high cells among CD4⁺CD25⁺ cells		Foxp3⁺ cells among CD4⁺CD25⁺ cells		FR4 high cells among CD4⁺CD25⁺ cells
	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)	%	# of cells (×10³)
2ip3n + hamster IgG	52.1±2.6	3.6±0.4	56.5±8.8	7.6±2.7	19.8±1.5	28.6±7.3	83.6±3.3	126.0±33.2
2ip3n + mAb 2.11	55.8±2.0	5.0±0.4	60.1±2.4	6.6±0.8	19.0±4.8	20.2±3.8	75.0±2.3	95.6±14.1
2ip3n + mAb UC3	53.5±2.6	11.1±5.7	60.6±1.1	13.6±5.6	21.4±3.0	17.0±2.5	77.3±1.1	60.4±16.2

Mice were treated as described in Fig. 1. 48 h after the last OVA-challenge, cells were isolated, passed over nylon wool columns and non-adherent fractions stained with antibodies specific for CD3ε, CD4, CD25 and intra-cellular Foxp-3 protein or cell-surface-expressed folate receptor 4. Cell frequencies were determined cytofluorimetrically as described in 2A. Absolute cell numbers (total cells/mouse) were obtained by multiplying the cell frequencies (percentage of total or Foxp3⁺ or FR4⁺, CD4⁺CD25⁺CD3ε⁺ T cells) by the number of cells obtained in lung digests. Results for each group are expressed as mean ± SEM (n = 4 in each group).