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. Author manuscript; available in PMC: 2015 Sep 30.
Published in final edited form as: Nat Commun. 2015 Mar 30;6:6641. doi: 10.1038/ncomms7641

Figure 5. CDK8 is a downstream effector responsible for Skp2-mediated cell growth, G2/M and polyploidy.

Figure 5

(a) The mRNA levels of CDK8 were measured by real-time PCR in MDA-MB-231 and BT474 cells with Luc or Skp2 knockdown (n=3). (b-d) Immunoblotting of MDA-MB-231 cells infected with pBabe or pBabe-Skp2 (B), infected with Luc or Skp2 shRNAs (c) or infected with Luc shRNAs, Skp2 shRNAs, or Skp2 plus mH2A1 shRNAs (d). (e) Immunoblotting assay (Left panel) and cell growth assay by direct cell counting (Right panel) of primary WT and Skp2−/− MEFs with stably expressing pBabe or pBabe-CDK8. (f) Flow cytometry analysis of DNA content in primary WT and Skp2−/− MEFs with stably expressing pBabe or pBabe-CDK8. (g) G2/M phase was determined by Flow cytometry analysis of primary WT MEFs with stably expressing pBabe or pBabe-CDK8. (h) Polyploidy of primary WT and Skp2−/− MEFs with stably expressing pBabe or pBabe-CDK8. (Error bars indicate s.e.m. Data represent mean values of three independent experiments. Student’s t-test used; *p<0.05)