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. Author manuscript; available in PMC: 2015 Jul 13.
Published in final edited form as: Nat Med. 2010 Sep 19;16(10):1120–1127. doi: 10.1038/nm.2213

Figure 3. CL disrupts lung structure and alters epithelial cell viability.

Figure 3

a. MicroCT scan images were obtained on live mice (in vivo) 1 h after i.t. administration of CL (50 nmole, [low], 100 nmole [high]) vs. control mice (above panels). In separate studies, mice were given CL as above and lungs were fixed and processed for microCT scanning (ex vivo). Data represents two mice/group. Below, third row: fixed tissue was processed for H & E staining (20× magnification). Lower row (middle) shows a high magnification (100×) image of foamy cells. b-c. CL induces lung edema. Mice were deeply anesthetized and administered CL (15 mM in 50 μl saline, i.t.; n = three) or vehicle (50 μl saline; n = 3). 30 min after CL administration Evan’s Blue dye was injected intravenously. Wet/dry weights of lungs were also determined (c). ***P<0.01 vs. control. d. Primary mouse type II cells were serum starved for 24 h and then exposed to Infasurf (120 nmol/ml) or CL (5-15 Mol%) for various times prior to harvest for detection of poly(ADP-ribose) polymerase (PARP) cleavage (d, arrows, left panel); mice were also given i.t. CL (50 nmol) for analysis of lung DNA fragmentation using a DNA ladder extraction kit ([BioVision], d, right panel) or assayed for cell viability (e) and LDH release (f, primary type II cells). NP-40 was used as a positive control for LDH release. Data represents three separate experiments where *P<0.05, **P<0.01, and ***P<0.001 vs. control as determined by one-way ANOVA analysis.