Fig. 4.

Compound 1 decreases neutrophil accumulation in mouse lungs. (A) Mice received oropharyngeal bleomycin on day 0 to induce acute respiratory distress syndrome. The control mice received saline. Mice were then treated with i.p. injections of 0.1 mg/kg of compound 1 (C1) or an equal volume of vehicle control on days 1 and 2. On day 3, the mice were euthanized and lungs after BAL were stained for the neutrophil marker Ly6G. Images are representative of three independent experiments. (Scale bar: 100 µm.) (B) BAL cells were stained for the indicated markers, n = 4. (C) After collecting the BAL, lungs were stained for the indicated markers, n = 4. *P < 0.05 (one-way ANOVA, Holm–Bonferroni post hoc test). (B and C) Values are mean ± SEM.