Fig. S7.

Compound 1 inhibits fibrocyte differentiation through SIGN-R1 without causing extensive cell death. Human PBMCs (A) or C57BL/6 mouse spleen cells (B) were incubated with the indicated concentrations of compound 1. After 5 d, Alamar blue was used to estimate cell viability, n = 3. (C) Mouse spleen cells were incubated with increasing concentrations of compound 1. After 5 d, cells were fixed, stained, and fibrocytes were counted, n = 4. Compound 1 inhibited murine fibrocyte differentiation with an IC₅₀ of 91 ± 47 pg/mL. *P < 0.05, **P < 0.01 (t test). All values are mean ± SEM. (C) C57BL/6 data were fit to a sigmoidal dose–response curve with a variable Hill coefficient.