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Monitoring vesicle formation. MLVs with encapsulated HPTS were incubated in 120 μM CuSO4, 60 μM hydroquinone, 350 mM TES, 60 μM tripropargylamine, 615 μM alkyne lysolipid, and 1.01 mM alkyl azide. Small daughter vesicles are highlighted with arrows. Numbers indicate elapsed time, in minutes, from start of experiment. (Scale bar, 5 μm.)
