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. 2015 Jun 22;112(27):8338–8343. doi: 10.1073/pnas.1424705112

Fig. 3.

Fig. 3.

BEND3 associates with NoRC. (A) BEND3 interacts with Tip5. Immunoprecipitation was performed using GFP antibody in cells expressing HA-Tip5 in the presence or absence of YFP-BEND3. (B) Reciprocal immunoprecipitation using T7 antibody was performed in cells expressing YFP-BEND3 with or without T7-Tip5. (C) Immunoprecipitation using HA antibody in cells stably expressing HA-BEND3. Note that BEND3 associates with endogenous NoRC (Tip5). (D) Tip5 occupancy at rDNA promoters in control (Scr) and BEND3-depleted cells (shBEND3). (E) HA-BEND3 occupancy at rDNA promoters in control (Gl3) and Tip5-depleted cells (si-1, si-2). ChIP data are represented as percentage of input. Error bars represent SD; n = 3. *P < 0.05, **P value < 0.01.