Fig. S4.

PAX5-dependent mechanisms of MuLV regulation. (A) ChIP assays for SETDB1 (Left) and H3K9me3 (Right) at LTRs for the indicated specific ERV proviruses. Assays were performed in Abl pro-B cells or ESC 4 d after treatment with tamoxifen (Δ/Δ) or vehicle (C/C). Results are representative of three independent experiments, and data are shown as mean ± SD (*P < 0.05, Student t test). (B) shRNA knockdown of Pax5 in Abl pro-B cells (Fig. 4D). Shown is a Western blot analysis using either PAX5- or GAPDH-specific antibodies. (C) SETDB1 mRNA expression (qRT-PCR) and protein binding (ChIP) following shPax5 knockdown. Results represent two independent experiments, and data are shown as mean ± SD.