Fig. S3.

Ectopic synthesis of the AlpR-CTD is toxic in cells of clinical P. aeruginosa isolates, and causes a decrease in culture density and an increase in DNA in the culture supernatant of strain PAO1. (A) Ectopic synthesis of the AlpR-CTD results in cell death or the inhibition of cell growth in P. aeruginosa clinical isolates CF18 and PA14. CF18 and PA14 cells containing pAlpR-CTD were serially diluted and incubated on media lacking or containing IPTG. (B) Ectopic synthesis of the AlpR-CTD results in cell death or the inhibition of cell growth in the Liverpool Epidemic Strain, LESB58. LESB58 cells containing pL-AlpR-CTD (in which synthesis of the AlpR-CTD is under the control of a weaker promoter than in pAlpR-CTD) or the empty control vector (pEV) were streaked and incubated on media lacking or containing IPTG. (C) In wild-type PAO1 cells grown in liquid culture, induction of the AlpR-CTD resulted in a decrease in the OD₆₀₀ of the culture over time that corresponded with an increase in the abundance of DNA in the culture supernatant (AlpR-CTD⁺), in comparison with wild-type cells that contained the empty vector control (pPSPK; AlpR-CTD⁻). Experiments were repeated three times and a representative dataset is shown.