Fig. S5.

Repression of alp promoter-lacZ fusions by AlpR and activation of the alpB promoter-lacZ fusion by AlpA. (A) Ectopic synthesis of AlpR in E. coli resulted in repression of alpR promoter– and alpA promoter–lacZ reporter fusions, whereas ectopic synthesis of λCI did not. (B) Ectopic synthesis of AlpR in P. aeruginosa resulted in repression of an alpB promoter–lacZ reporter fusion, but did not repress a control orn promoter–lacZ reporter fusion. Plasmid pPSV38 served as an empty vector control. (C) Ectopic synthesis of AlpA in E. coli resulted in activation of an alpB promoter–lacZ reporter fusion, whereas activity did not increase in cells that contained the empty vector. Error bars denote SD from the mean.