Fig. S7.

Cells lacking alpBCDE tolerate ectopic synthesis of the AlpR-CTD and ectopic synthesis of alpBCDE results in lethality. (A) Cells lacking alpBCDE tolerate ectopic synthesis of the AlpR-CTD almost as well as cells that lack alpA. Wild-type PAO1 cells, cells of the PAO1 ΔalpA mutant strain, and cells of the PAO1 ΔalpBCDE mutant strain containing pAlpR-CTD were serially diluted and incubated on media lacking or containing IPTG. (B) Ectopic synthesis of alpBCDE results in lethality in strain PAO1. Wild-type PAO1 cells containing the empty vector pPSV38 or the alpBCDE expression plasmid pAlpBCDE were serially diluted and incubated on media lacking or containing IPTG.