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. 2015 Jun 22;112(27):8409–8414. doi: 10.1073/pnas.1500223112

Fig. 2.

Fig. 2.

MET expression in KB1P chimeric mice leads to accelerated mammary tumor development. (A) Overview of the experimental procedure. Female ESCs derived from our KB1P mouse model were equipped with a Col1a1-frt homing cassette, and subsequently the Met proto-oncogene was introduced by recombinase-mediated cassette exchange. (B) Scatter plot illustrating the performance of KB1P ESC clones targeted with the Col1a1-frt homing cassette (Col1a1), and additionally equipped with Met (MET clone A9 and B3) to produce chimeric mice. (C) Mammary tumor-free survival of KB1P chimera (red; t50 = 284 d, n = 13 mice), KB1P-Col1a1 (black; t50 = 299 d, n = 14 mice), and KB1P-MET (blue; t50 = 176 d, n = 11 mice) chimeric mice. Log-rank (Mantel–Cox) P values are indicated. (D) Immunohistochemical staining of formalin-fixed/paraffin-embedded sections from KB1P, KB1P-Col1a1, and KB1P-MET chimeric mammary tumors for H&E, MET, METY1234/1235, E-cadherin, and vimentin. (E) Pie diagrams illustrating the distribution of different mammary tumor types in KB1P (93% carcinoma; 7% carcinosarcoma), KB1P-Col1a1 (100% carcinoma), and KB1P-MET (45% carcinoma; 55% carcinosarcoma) chimeric mice. Carcinomas are shown in blue and carcinosarcomas in orange. (F) Box plot showing relative expression of claudin 3, 4, and 7 in KB1P-MET carcinomas and carcinosarcomas. Mann–Whitney P values are indicated.