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Gating strategy in the transduced thymocyte OP9-DL4 stromal cell culture system. N15β WT or vector-only transduced thymocytes were cultured with OP9 parental or OP9-DL4 stromal cells. Day 6 of culture is shown. Gating strategy is shown at the top, and numbers represent percentages in designated quadrants or circumscribed regions of the dot plots.
