Table S1.
Mutants used in cellular and NMR experiments
| TCRβ* | Name | Region | Changes† |
| N15 | C domain | Cβ–Cα interface | F126R/V142Q/L144Q |
| N15 | M22 | CDR2 | Y50A/E51A |
| N15 | M23 | CDR2 | Q48A/Y50A/E51A |
| N15 | W97A | CDR3 | W97A |
| N15 | MP1 | Vβ patch | F103A |
| N15 | MP2 | Vβ patch | L43A/F103A |
| N15 | MP3 | Vβ patch | Q37A/L43A/F103A |
| N30 | C domain | Cβ–Cα interface | F127R/V143Q/L145Q |
| N30 | M1 | CDR1 | S27A/H29A/D30A |
| N30 | M2 | CDR2 | D51A/E52A |
| N30 | M3 | CDR3 | S96G/Δ(G97-V102) |
| N30 | M12 | CDR1/2 | M1 + M2 |
| N30 | M123 | CDR1/2/3 | M1 + M2 + M3 |
*
For cellular transductions, all mutants are derived from WT β. For NMR studies, N15β WT and mutants M22, M23, and W97A are derived from the C-domain mutant F126R/V142Q/L144Q, and N30β refers to the C-domain mutant F127R/V143Q/L145Q.
†
Numbering according to Fig. S1.