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. 2015 Jun 23;112(27):8314–8319. doi: 10.1073/pnas.1505421112

Fig. 5.

Fig. 5.

The interplay of ECM stiffness and cell contractility regulates SRp40 phosphorylation. (A) Fluorescent staining for phosphorylated SR protein of ECs plated on compliant (E = 1 kPa) and stiff (E = 10 kPa) substrates. (B) Western blot of whole protein extract from ECs plated on either compliant (E = 1 kPa) or stiff (E = 10 kPa) substrates showing an increase in SR protein phosphorylation with increasing substrate stiffness. (C) Western blot of whole protein extract from EC cells plated on stiff (E = 10 kPa) substrates showing that Y27632-mediated inhibition of cell contractility prevent SR protein phosphorylation compared with control (Ctrl). Actin was used as a loading control. (D) Representative image of frozen tissue sections stained for phosphorylated SR protein and CD31 showing that the BAPN treatment lowered SR protein phosphorylation compared with the mock treatment (Ctrl) (images were acquired with identical exposure settings). (Scale bars, 30 μm.)