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. 2015 Jun 23;112(27):8314–8319. doi: 10.1073/pnas.1505421112

Fig. S2.

Fig. S2.

ECM stiffness regulates the expression of the PKC βII and VEGF 165b isoforms. (A) Representative confocal images of frozen tissue sections from PyMT tumors with or without BAPN treatment and stained for PKC βII showing the overall increase of the PKC βII isoform with tumor tissue stiffness. (B) Representative confocal images of frozen tissue sections from PyMT tumors with or without BAPN treatment stained for VEGF 165b and total VEGF showing the overall decrease of the VEGF 165b isoform in stiffer tumor tissue. (C) Representative Western blot of total protein extract from ECs in culture on compliant and stiff substrates with the corresponding densitometric quantification showing increased PKC βII isoform expression. Actin was used as loading control. (D) Representative Western blot of total protein extract from ECs in culture on compliant and stiff substrates with the corresponding densitometric quantification showing increased VEGF 165b isoform expression. GAPDH was used as loading control. Plots are mean ± SE, Student t test: ***P < 0.001. (Scale bars, 30 μm.)