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Longer exposure of GST blot from Input image of Fig. 2B. GST or the indicated GST-GADD34 fusion protein was overexpressed in the yeast strain YM100 that expresses human eIF2α and human PP1. WCEs [5% (wt/vol) of the input samples used for GST pull-down assays] were subjected to SDS/PAGE and immunoblot analysis by using antibodies against GST, the Myc-epitope on eIF2α, or PP1.
