Fig. 1.

Bam35 DNA polymerase synthetic and degradative activities. Shown is denaturing PAGE analysis of primer extension assays with a matched (A and C) or mismatched (B and D) 3′-terminus as depicted above the gels. The assays were carried out for 10 min at 37 °C in the presence of 10 nM of either WT (B35DNAP, A and B) or exonuclease-deficient (B35DNAPexo^—, C and D) DNAPs and the indicated concentration of dNTPs. Positions of the 15-mer substrate and 33-mer product bands are indicated on the right.