Skip to main content
. 2015 Jun 22;112(27):E3476–E3484. doi: 10.1073/pnas.1510280112

Fig. 2.

Fig. 2.

Bam35 DNA polymerase can couple strand displacement and processive polymerization during rolling circle DNA replication. (A) Replication of primed M13 DNA was carried out as described in Materials and Methods in the presence of 40 μM each of the four dNTPs and 2.25–91 nM of B35DNAP (lanes 1–9) or 50 nM of Φ29DNAP (lane 11). After incubation at 37 °C for 20 min, the length of the synthesized DNA was analyzed by alkaline 0.7% agarose gel electrophoresis alongside a λ DNA ladder (lane 10) and autoradiography. M13 ssDNA unit length is indicated as well. (B) Multiple-displacement amplification of plasmidic DNA by B35DNAP. The assay was performed as described in Materials and Methods, in the presence of 0–10 ng of pUC19 plasmid DNA as input and 50 nM of B35DNAP (lanes 2–9). Linearized pUC19 plasmid (100 ng) was loaded in lane 1, and linear DNA fragments obtained after digesting Φ29 DNA with HindIII, used as DNA length markers (lane 10), are indicated on the right.