Fig. 7.

Short-term memory of abasic sites containing mismatch by B35DNAP. (A) Detailed template strand sequence as well as the alternative primers used are depicted above the gel, where “X” represents either T or THF nucleotide. The relative distance of the X with respect to the primer size is also indicated on the right. Reactions were performed for 10 min at 37 °C in the presence of 10 nM B35DNAP and 100 μM dATP (lanes 9, 11, 16, and 18), dATP and dCTP (lanes 8, 12, 14, 15, 19, and 21), dATP and dGTP (lanes 10 and 17), or dCTP and dGTP (lanes 13 and 20). (B) B35DNAP replication of the same primer/template substrates but with 10 µM of the four dNTPs. Primer length is specified above the gel and positions of the 15- and 22-mer substrates and the 16- and 33-mer products are indicated on the right.