Fig 8.

The B35DNAP TPR2 motif is required for extension beyond the abasic site. Polymerization activity of WT (10 nM) and ΔTPR2 (200 nM) B35DNAP on primer/template substrates with or without a THF lesion, as depicted above. Reactions were performed for the indicated times at 37 °C in the presence of 100 μM dNTPs. Positions of the 15-mer substrate and the 16- and 33-mer products are indicated on the right.