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Effect of dNTP concentration on B35DNAP abasic site TLS. Primer extension assays of the 3′-GFT sequence context are shown. The assays were carried out for 10 min at 37 °C in the presence of 10 nM of either WT (A) or exonuclease mutant (B) B35DNAP and the indicated concentration of all dNTPs. Positions of the 15-mer substrate and 33-mer product bands are indicated on the right.
