Table S1.
Oligonucleotide | Sequence |
OL15 (15 mer) | 5′-GATCACAGTGAGTAC-3′ |
OL15MM (15 mer) | 5′-GATCACAGTGAGTAG-3′ |
OL16 (16 mer) | 5′-GATCACAGTGAGTACA-3′ |
OL19 (19 mer) | 5′-GATCACAGTGAGTACAATA-3′ |
OL21 (21 mer) | 5′-GATCACAGTGAGTACAATAGA-3′ |
OL22 (22 mer) | 5′-GATCACAGTGAGTACAATAGAA-3′ |
OL23 (23 mer) | 5′-GATCACAGTGAGTACAATAGAAC-3′ |
OL25 (25 mer) | 5′-GATCACAGTGAGTACAATAGAACGA-3′ |
OLC33GTT (33 mer) | 5′-ACTGGCCGTCGTTCTATTGTACTCACTGTGATC-3′ |
OLC33GFT (33 mer) | 5′-ACTGGCCGTCGTTCTATFGTACTCACTGTGATC-3′ |
OLC33GTA (33 mer) | 5′-ACTGGCCGTCGTTCTAATGTACTCACTGTGATC-3′ |
OLC33GFA (33 mer) | 5′-ACTGGCCGTCGTTCTAAFGTACTCACTGTGATC-3′ |
B35pol-fw | 5′-CGCGAAGCTTAAAGGAGGAAGCATATGATGACTACTACTAATAGAAAAAAGCGT-3′ |
B35pol-rv | 5′-CCGGATCCTTATTATAAGAAACTTAATTCACCTAATAGTTCTTCAT-3′ |
Exo-fw | 5′-CTTTACGCTAGCTACTGCAACACGTGGTTTGGACGGCGCCGTGTTTCGAA-3′ |
Exo-rv | 5′-TTCGAAACACGGCGCCGTCCAAACCACGTGTTGCAGTAGCTAGCGTAAAG-3′ |
ΔTPR2-fw | 5′-TGGAAAATTTGCAATGCAGCGGGAATATGCTAAGGCTGAATATATTCAAC-3′ |
ΔTPR2-rv | 5′-GTTGAATATATTCAGCCTTAGCATATTCCCGCTGCATTGCAAATTTTCCA-3′ |
LacZFW | 5′-AGCTTGTCTGTAAGCGGATGCCG-3′ |
F represents the THF abasic site analog. Names of complementary 33-mer oligonucleotides include three letters corresponding to the -1, +1, and +2 template bases in the direction of the polymerization.