Table 1.

Determination of error rate values for B35DNAP during MDA

Experiment	Total colonies	f	f0	d	Error rate
1	1,171	1.8 × 10−2	5.7 × 10−3	11.2	3.2 × 10−6
2	10,605	2.7 × 10−2	4.6 × 10−3	9.9	6.5 × 10−6
3	10,682	2.1 × 10−2	2.2 × 10−3	10	5.6 × 10−6
Average ± SD		1.9 ± 0.2 × 10−2	4.2 ± 1.6 × 10−3	10.4 ± 0.9	5.1 ± 1.7 × 10−6

Mutant frequencies (f) were determined by dividing the total number of white plaques by the total number of plaques. f₀ represents the background frequency in plasmid DNA purified from bacteria, digested and religated in the same conditions (Materials and Methods). Template doublings (d) were calculated using the equation d = (ng product)/(ng input). Error rate was calculated using the equation ER = (f-f₀)/bp × d, where bp is the number of detectable sites in the lacZ gene (342 bp). Data from three independent experiments, and average ± SD values are shown.