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. 2015 Jun 22;112(27):8451–8456. doi: 10.1073/pnas.1502948112

Fig. 2.

Fig. 2.

RtcB inhibits axon regeneration independently of its role in tRNA ligation. (A) tRNAs containing introns are cleaved by the splicing endonuclease complex containing TSEN2. The resulting tRNA halves are ligated by RtcB and its cofactor archease. (B) Percentage of axons that regenerated back to the DNC after 24 h. Error bars indicate 95% confidence interval. Number of axons cut per genotype from left to right: 47, 26, 30, 18, 15, 38, and 24. (C) Representative micrographs 96 h after egg collection of WT, rtcb-1(gk451), tsen-2(wp15), and arch-1(wp16) with or without prespliced tRNAs. (Scale bar: 500 μm.) (D) Quantification of body length measurements at 96 h after egg collection (20 animals per genotype). Error bars indicate SEM. NS, not significant; ***P < 0.0005.