Table 2.
Residues with NMR Signals Sensitive to Environmental Conditions
| Apo proteina | Cofactor substitutionb | Temperature variationc | Urea titrationd | Relative reagent accessible surface area (%)e | |
|---|---|---|---|---|---|
| A46 | X | X | X | 0 | |
| scW47 | X | X | 67f | ||
| N48 | X | X | 22 | ||
| F71 | X | X | 0 | ||
| G72 | X | X | 0 | ||
| A74 | X | X | X | 0 | |
| G159 | X | X | X | X | 79 |
| F160 | X | X | 0 | ||
| S193 | X | X | X | 0 |
a
Backbone 15N/1H resonance absent in spectra of apo-NOX and present in holo-NOX.
b
> 0.1 ppm, comparing FMN- and FAD-saturated NOX spectra.
c
|∂δN/∂T| > 17 ppb/K or ∂δH/∂T < −4.5 ppb/K.
d
Δδ > 0.3, comparing NOX spectra obtained with 0 and 2M urea.
e
Amide NH accessibility based on a 2.5 Å probe.
f
Indole NH accessibility based on a 2.5 Å probe.