Figure 6.

Effect of the N-terminus of the β-strand on corepressor affinity for Rev-erbα LBD. SmrtID1B-2-rhodamine competed with increasing concentrations of full-length NcorID1C (0, 2.5, 5, 10 μM); (B) full-length SmrtID1C (0, 2.5, 5, 10 μM); (C) NCoRD1B-2 (0, 10, 50, 100 μM); (D) SmrtID1B-2 (0, 10, 50, 100 μM). Graphs in (A) and (C) are redrawn from Figure 2 for comparison. Titration of the labeled peptide by Rev-erbα LBD in the absence of competitor peptide is in pink. Increasing concentrations of competitor peptide are blue, yellow and green, respectively.