Figure 3.

Monoamine release. Monoamine release was induced by a high concentration of the compound (100 μM) after preloading the transporter-transfected cells with the respective radiolabelled monoamine. All of the benzofurans released NA, dopamine and 5-HT similarly to methamphetamine and MDMA. In contrast, the benzodifuran 2CB-FLY was not a monoamine releaser. Transporter blockers induced non-specific ‘pseudo-efflux’ (horizontal dashed line, open bars), which arises from substrate that diffuses out of the cells and from subsequent reuptake inhibition. Compounds that produced significantly more monoamine efflux (*P < 0.05, **P < 0.01, ***P < 0.001) compared with the respective non-releasing uptake inhibitors (negative controls, open bars) were considered monoamine releasers. The data are expressed as the mean ± SEM of three to four independent experiments.