Table 1. Primers and PCR conditions used for P. larvae 16S-PCR identification, rep-PCR analysis and PlyPl23 cloning.

Amplicon	Sequence (5’- 3’)	Annealing (oC.s-1)	Extension (s)
16S rRNA	F: CTTGTGTTTCTTTCGGGAGACGCC; R: TCTTAGAGTGCCCACCTCTGCG	55/30	90
Rep-PCR	ERIC2: AAGTAAGTGACTGGGGTGAGC; ERIC1R: ATGTAAGCTCCTGGGGATTCA	47/45	120
PlyPl23	Ply23F: AGAAGCCATATGATGGAAATCAGACAGATGCTAGTAGGA (NdeI); Ply23R: TACGATGGATCCTCACAGGCACCCACTCGCCAAAA (BamHI)	53/30	60