Figure 8.

Knockdown S6 by siRNA transfection reduces the expression of GLS and activity of GDH. HEY cells were transfected with RPS6 siRNA for 24 h. Western blotting showed the expression of phosphorylation of S6 was inhibited after siRNA transfection (A). HEY cells were treated with 2.0 mM glutamine for 24 h after siRNA transfection. Both of S6 siRNA and rapamycin reduced the expression levels of GLS and phosphorylation of S6 (B). S6 siRNA transfection decreased GDH activity induced by glutamine (C). HEY cells were treated with 2.0 mM glutamine for 48 h after siRNA transfection. Cell proliferation was determined by MTT assay (D). *P<0.05.