Fig 8. Amplified products from reverse-transcribed cDNA obtained from Symbiodinium kawagutii cultures co-incubated with Agrobacterium tumefaciens harboring pCB302-gfp-AtRACK1C, pCB302-gfp-MBD, and pCB302-gfp-FABD2.

The reverse-transcribed cDNA from S. kawagutii cultures expressing the gfp-fusion constructs was used as template with the gfp primers for PCR amplification of the corresponding transcripts (see Materials and Methods). Fragments of ~0.7 kbp corresponding to the gfp transcripts were obtained from cDNA of S. kawagutii co-incubated with A. tumefaciens harboring: gfp-AtRACK1C (lane 3), gfp-MBD (lane 5), or gfp-FABD2 (lane 6). No amplifications were obtained when template cDNA was used from the following negative S. kawagutti controls: no shaking/no Agrobacterium (lane 1); shaking/no Agrobacterium (lane 2); or Agrobacterium/no shaking (gfp-AtRACK1C) (lane 7). The presence of cDNA in preparations from control and A. tumefaciens co-incubated cells was confirmed by RT-PCR of the endogenous S. kawagutii RACK1 transcript (data not shown). Lane 4 shows the molecular standards. The bands on the gel are shown in negative.