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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Cell Rep. 2015 Jun 25;12(1):140–149. doi: 10.1016/j.celrep.2015.06.003

Figure 5. Ebolavirus nucleoprotein RNA binding.

Figure 5

The potential of different NP N-terminal domain constructs to bind a FITC-labeled 18 nucleotide RNA was assessed. Binding was measured by changes in the fluorescence anisotropy of the FITC-RNA with increasing protein concentrations. The oligomeric NP 1-450 (squares) shows 50% binding at a protein concentration of ~20 nM. No appreciable binding is observed for the monomeric NP°-VP35 complex (circles, VP35 15-60-NP34-367 protein fusion) or for the monomeric NP° core (triangles, NP 34-367).