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. 2015 Jul 13;10(7):e0132441. doi: 10.1371/journal.pone.0132441

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 2 — Samples were analyzed on 1% agarose gel in 0.5X TBE buffer. Lane M: 1 Kb DNA ladder (Merck, India); Lane 1: Garden soil; Lane 2: Sewage sludge; Lane 3: Lake soil; Lane 4: Compost; Lane 5: E.coli MTCC 40; Lane 6: Bacillus subtilis NRRL-B-201. Lane 7: Negative control. (B). Gel electrophoresis of PCR amplified ITS-2 for the DNA extracted using the powdered glass method M6. Lane M: 100bp DNA ladder (Merck, India); Lane 1: Negative control; Lane 2: PCR amplification of ITS -2 region of Chlorella sorokiniana UTEX# 1666.